ECLectic Network News

Meet our doctoral candidate Tereza Jahodová

Meet our doctoral candidate: Tereza Jahodová from Czech Republic 🇨🇿

As part of the ECLectic network, she will focus on the point of care microfluidic tool development at Dynamic Biosensors (Germany 🇩🇪) under the supervision of prof. Alice Soldà.

She recently graduated at Uppsala University in Sweden, where she took an interest in investigating the biophysical properties of molecular interactions using the switchSENSE technology.

If you want to know more, click the button below and read her full bio and the description of her doctoral project in the dedicated section.


Tunable electrochemiluminescence of TADF luminophores: manipulating efficiency and unveiling water-soluble emitters

New paper in Chemical Science

Tunable electrochemiluminescence of TADF luminophores: manipulating efficiency and unveiling water-soluble emitters

Alessandro Fracassa, Francesco Calogero, Giulio Pavan, Pavlos Nikolaou, Andrea Fermi, Paola Ceroni, Francesco Paolucci, Pier Giorgio Cozzi, Thomas Scattolin, Nicola Demitri, Fabrizia Negri, Andrea Gualandi, Alessandro Aliprandi and Giovanni Valenti

Published in Chemical Science

 

Abstract
Thermally Activated Delayed Fluorescent (TADF) luminophores offer the potential to achieve 100% Internal Quantum Efficiency (IQE) by harvesting both singlet and triplet excitons via reverse intersystem crossing from T1 to S1. This class of molecules has therefore been embraced in the pursuit of cheaper and more efficient electrochemiluminescent (ECL) labels. The present study explores how tuning the electron-donating (D) and -accepting (A) strengths of peripheral substituents affects the ECL emission of mono- and dicyanoarene-based TADF dyes. To this end, we synthesized two series of TADF compounds, independently manipulating electron donors and acceptors by (i) halogenating electron-rich diphenylamine moieties, or (ii) mono- or di-substituting the electron-poor cyanoarene core with either fluorine or imidazole. Through a comparative analysis, we elucidate the role of each substituent in shaping the photophysics of the investigated luminophores. Despite only achieving a relative ΦECL as high as 1.27%, this framework identifies several molecular features that boost the ECL efficiency to pave the way for designing highly efficient TADF-based ECL emitters. Ultimately, imidazole substituents are exploited as a platform for functionalization with triethylene glycol units. The resulting water-soluble TADF luminophores are characterized under conditions usual to commercial ECL bioanalysis, proving their potential as a cost-effective alternative replacement to [Ru(bpy)3]2+ in clinical diagnostic.
Tunable electrochemiluminescence of TADF luminophores: manipulating efficiency and unveiling water-soluble emitters
Fig. 1 Molecular structures of three different scaffolds, namely 3DPABN, 3DPAIPN, and 4DPAIPN. The first two molecular platforms underwent further modification by derivatizing the cyanoarene accepting core with fluorine atoms (i.e., 3DPA2FBN and 3DPAFIPN), imidazole heterocycles (i.e., 3DPA2ImBN and 3DPAImIPN), or imidazole units bearing a triethylene glycol chain to provide water solubility (i.e., [3DPA2(ImTEG)BN](OTs)2 and [3DPA(ImTEG)IPN]OTs). On the other hand, the functionalization of 4DPAIPN focused on derivatizing the electron-donating DPA groups, which were subjected to mono- or bi-insertion of bromine or chlorine atoms (i.e., 4(BrDPA)IPN, 4(Br2DPA)IPN and 4(Cl2DPA)IPN).


ECLectic Network News

Meet our doctoral candidate Abdulkadir Sadiq

Meet our doctoral candidate: Abdulkadir Sadiq from Nigeria 🇳🇬

He will investigate the analytical performances of a lab-on-a-chip device for bacteria quantification at Albert-Ludwigs-Universität Freiburg (Germany 🇩🇪) under the supervision of prof. Siegbert Rieg.

He recently completed his master’s degree in Biotechnology at the Universiti Malaya in Malaysia with a thesis focused on the anti-virulence potential of a synthesized compound against Staphylococcus aureus.

If you want to know more, click the button below and read his full bio and the description of his doctoral project in the dedicated section.


ECLectic Network News

Meet our doctoral candidate Stephania Rodríguez Muiña

Meet our doctoral candidate: Stephania Rodríguez Muiña from Spain 🇪🇸

She will work on developing an integrated microfluidic platform for the multiplex detection of sepsis biomarkers at BCMaterials (Spain 🇪🇸) under the supervision of Professor F. Javier del Campo García.

Recently she developed a flexible electrochemical biosensor platform for cell culture monitoring at the CRNL and Laboratoire Ampère in Lyon.

If you want to know more, click the button below and read her full bio and the description of her doctoral project in the dedicated section.


AnalBioChem_sept24_FrontespizioNews

New paper in Analytical and Bioanalytical Chemistry

Biosensing strategies using recombinant luminescent proteins and their use for food and environmental analysis

Fernando Pradanas-González, Marta García Cortés, Bettina Glahn-Martínez, Melisa del Barrio, Pablo Purohit, Elena Benito-Peña and Guillermo Orellana

New paper in Analytical and Bioanalytical Chemistry

Abstract

Progress in synthetic biology and nanotechnology plays at present a major role in the fabrication of sophisticated and miniaturized analytical devices that provide the means to tackle the need for new tools and methods for environmental and food safety. Significant research efforts have led to biosensing experiments experiencing a remarkable growth with the development and application of recombinant luminescent proteins (RLPs) being at the core of this boost. Integrating RLPs into biosensors has resulted in highly versatile detection platforms. These platforms include luminescent enzyme-linked immunosorbent assays (ELISAs), bioluminescence resonance energy transfer (BRET)–based sensors, and genetically encoded luminescent biosensors. Increased signal-to-noise ratios, rapid response times, and the ability to monitor dynamic biological processes in live cells are advantages inherent to the approaches mentioned above. Furthermore, novel fusion proteins and optimized expression systems to improve their stability, brightness, and spectral properties have enhanced the performance and pertinence of luminescent biosensors in diverse fields. This review highlights recent progress in RLP-based biosensing, showcasing their implementation for monitoring different contaminants commonly found in food and environmental samples. Future perspectives and potential challenges in these two areas of interest are also addressed, providing a comprehensive overview of the current state and a forecast of the biosensing strategies using recombinant luminescent proteins to come.

 

AnalBioChem_sept24_FrontespizioNews


ECLectic Network News

Meet our doctoral candidate Wissal El Majdoub

Meet our doctoral candidate: Wissal El Majdoub from France 🇫🇷

She will work on developing a micro- and nano-channels platform for ECLectic at Stichting imec Nederland (The Netherlands 🇳🇱) under the supervision of Professor Klaus Mathwig.

She recently obtained a MEng degree in Chemistry from the Graduate School of Food Science, Materials Science, and Chemistry in Bordeaux (France 🇫🇷), where she focused on nano and microtechnologies, with a particular emphasis on addressing pressing health-related challenges.

If you want to know more, click the button below and read her full bio and the description of her doctoral project in the dedicated section.


ECLectic project has received funding from the European Union’s MSCA Doctoral network Horizon Europe programme Grant Agreement Number 101119951.

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